Fluorescence of Lysozyme and Lysozyme Substrate Complexes
نویسندگان
چکیده
منابع مشابه
Tryptophan fluorescence lifetimes in lysozyme.
Tryptophan fluorescence lifetimes at pH 2 and pH 8 have been obtained for lysozyme and for lysozyme derivatives in which tryptophan-62 or tryptophan-108 or both are nonfluorescent. The lifetimes range from about 0.5 ns to 2.8 ns for the various emitting tryptophans. The tryptophan lifetimes appear to increase with exposure of tryptophan to solvent, but intramolecular contacts, probably with cys...
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Luminescence is the emission of photons from electronically excited states. The electron in the in the lowest excited singlet state (S1), has the opposite spin orientation as the second electron in the lower ground state (S0) orbital. The ground state and excited state electrons are said to be paired. Fluorescence is the emission which results from the return to the lower orbital of the excited...
متن کاملThe effect of salt on self-assembled actin-lysozyme complexes.
We present a combined experimental and computational study of the bundling of F-actin filaments induced by lysozyme proteins. Synchrotron small-angle x-ray scattering results show that these bundles consist of close-packed columnar complexes in which the actin is held together by incommensurate, one-dimensional arrays of lysozyme macroions. Molecular dynamics simulations of a coarse-grained mod...
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Effects of ?-cyclodextrin, ?CD, on refolding of lysozyme was investigated at pH 12 employing isothermal titration calorimetry (ITC) at 300K in 30mM Tris buffer solution. ?CD was employed as an anti-aggregation agent and the heats obtained for lysozyme+?CD interactions are reported and analyzed in terms of the extended solvation model. It was indicated that there are two sets of identical and no...
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Surface charge switch of a 6-carboxyfluorescein (FAM) labeled lysozyme aptamer from negative to positive upon lysozyme binding triggers fluorescence resonance energy transfer (FRET) between an anionic conjugated polymer and the aptamer, which provides a convenient method for lysozyme detection in biological media with high sensitivity and selectivity.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)99506-2